Neuroglia, edited by Helmut Kettenmann and Bruce R. Ransom, Third Edition, Oxford University Press 2012
THE ASTROCYTE TRANSCRIPTOME
Ditte Lovatt and Maiken Nedergaard
Center for Translational Neuromedicine
University of Rochester
601 Elmwood Ave, Box 645
Rochester NY 14642
Table 28.S1: GO annotated astrocyte enriched genes (download)
Astrocyte enriched genes were submitted to DAVID http://david.abcc.ncifcrf.gov/ , and GO molecular annotations were extracted. Of the 311 genes, 57 did not retrieve a GO term, and is not included in this list. This astrocyte enriched gene list was produced by the extracting the intersection of astrocyte-enriched genes defined by a fold change ≥ log base 2 and a FDR-corrected p-value ≤ 0.05 among three previously published datasets (Lovatt et al., 2007, Cahoy et al., 2008, Doyle et al., 2008). Astrocyte-enriched genes from Lovatt et al. (2007) was produced by comparing adult cortical GFAP-GFP+/GLT1+ astrocytes to GFAP-GFP–/GLT1– cells (n=3). Astrocyte-enriched genes from Cahoy et al. (2008) was produced by comparing postnatal (P)16-17 fluorescence activated cell sorting- (FACS) and panning-isolated astrocytes (n=5) to P16-17 panning-isolated neurons (n=3). Astrocyte-enriched genes from Doyle et al.(2008) was produced by comparing TRAP affinity-purified mRNA from BAC-transgenic Aldh1l1 mice. From Doyle et al. (2008), each sample (n=4) derived from several 8-10 week old mice, and was compared to unbound affinity-purified samples (n=2). Each of the three datasets was normalized individually using the RMA algorithm in R/Bioconductor. A representative probe chosen for cases where a gene has more than one probeset for the platform. This was done by choosing the probeset with the lowest p-value for that gene. When there was a p-value tie, the probeset with the higher value fold change was chosen.
You can use the FIND function (CTRL-F) to search for words within the document.